核固紅染色液（0.2%）
貨號(hào)：G1321
規(guī)格：100ml
英文名稱 : NUCLEAR FAST RED
CAS : 6409-77-4

    核固紅染色液是組織切片染色中常用的復(fù)染液，染色后細(xì)胞核呈紅色。染色液可重復(fù)使用多次，但染色效果會(huì)逐漸降低。

核固紅染色液使用方法：

1、 切片脫蠟水，可進(jìn)行其他染色，染色后蒸餾水洗2-5min。

2、 將組織切片浸入核固紅染色液，也可以直接滴加到樣本上染色5-10分鐘(淺染細(xì)胞核)，染色時(shí)間可根據(jù)染色深度做相應(yīng)調(diào)整。

3 自來(lái)水中沖洗去除多余的染色液。

4、 常規(guī)脫水透明，中性樹膠封固。

注意事項(xiàng)：

1、 切片脫蠟應(yīng)盡量干凈。

2、*次使用本試劑時(shí)建議先取1-2個(gè)樣品做預(yù)實(shí)驗(yàn)。

3、染色過(guò)程推薦淺染，通常能夠分辨細(xì)胞核即可，顏色過(guò)深有可能影響細(xì)胞顏色。

4、為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Nuclear Fast Red
CAS Number: 6409-77-4
Storage Temperature: Room Temperature
Product Description :
Appearance: Dark Red to Very Dark Brown Powder
Molecular Formula: C14H8NNaO 7S
Molecular Weight: 357.3
Synonyms: Kernechtrot, Calcium Red
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain
solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used
as a reagent for calcium in tissue where it forms a scarlet lake. Lakes are also formed with lead, ferric
ion, copper, tin, and strontium, but not barium. The suitability of nuclear fast red as a nuclear stain in
autoradiographs of knee joints of mice and rats has been reported.
Storage/Stability :
When stored at room temperature, this product is given a shelf-life of four years from the date of
manufacture.
Preparation Instructions:
This dye is reported to be soluble in water (6 mg/ml) and in ethanol (0.4 mg/ml), but it is likely that heat will
be required for the aqueous solution.
Procedure :
Preparation of a nuclear fast red (Kernechrot) solution for use as a counterstain:
1. Dissolve 25 g of aluminum sulfate in 500 ml of distilled water.
2. Add 0.5 g of nuclear fast red. Use heat to dissolve.
3. Cool, filter, and add a few grains of thymol as a pretive.
For use as a counterstain:
1. Rinse sections in distilled water.
2. Counterstain sections in nuclear-fast red solution for 5 minutes.
3. Wash in running tap water for 1 minute or more.
4. If using an aqueous mounting medium, the slides are ready to coverslip. If using a permanent
mounting medium, dehydrate in 2 changes of 95% alcohol, 2 changes of absolute ethanol, and 2 changes
of xylene. Slides can now be mounted using a synthetic, permanent mounting medium.

Note: Inadequate washing of the slides after nuclear fast red staining will result in cloudy slides. If the
slides have not been coverslipped in a permanent mounting medium, return to running water and wash
well. If slides have been coverslipped in a permanent mounting medium, soak in xylene to remove thecoverslip, soak in 3 changes of xylene to remove the mounting 

核固紅染色液（0.2%）訂購(gòu)說(shuō)明：
1、絕大部分產(chǎn)品備有現(xiàn)貨，一般情況下都能訂貨當(dāng)日發(fā)貨。 
2、部分非常用產(chǎn)品，需提前1－2日預(yù)訂，海外期貨則需要提前3-6周預(yù)訂。 
3、部分產(chǎn)品價(jià)格會(huì)因貨期、批次等因素發(fā)生變化，若有變動(dòng)以訂貨當(dāng)日新價(jià)格為準(zhǔn)。 
4、每日訂單截止時(shí)間為16點(diǎn)整，部分城市可到17點(diǎn)整，因超過(guò)截止時(shí)間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
5、請(qǐng)辦理完貨款后，將收據(jù)、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。

運(yùn)輸說(shuō)明：
1、極低溫產(chǎn)品：極低溫產(chǎn)品運(yùn)輸過(guò)程中加裝干冰運(yùn)輸。用干冰把產(chǎn)品包裹起來(lái)，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
2、低溫產(chǎn)品：低溫產(chǎn)品運(yùn)輸過(guò)程中加裝索萊寶冰袋運(yùn)輸。事先用冰袋把產(chǎn)品包裹起來(lái)，再使用泡沫盒密封，用膠帶嚴(yán)實(shí)密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續(xù)一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
3、常溫產(chǎn)品：常溫產(chǎn)品運(yùn)輸過(guò)程中無(wú)需加冰或者特殊包裝。產(chǎn)品由公司庫(kù)房人員快速配貨，準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。